"Inducing aggresome and stable tau aggregation in Neuro2a cells with an optogenetic tool" by Shigeo Sakuragi, Tomoya Uchida, Naoki Kato, Boxiao Zhao, Toshiki Takahashi, Akito Hattori, Yoshihiro Sakata, Yoshiyuki Soeda, Akihiko Takashima, Hideaki Yoshimura, Gen Matsumoto, Hiroko Bannai is published in BPPB as the J-STAGE Advance Publication.
2024 October 29 BPPB
A following article is published as the J-STAGE Advance Publication in "Biophysics and Physicobiology".
Shigeo Sakuragi, Tomoya Uchida, Naoki Kato, Boxiao Zhao, Toshiki Takahashi, Akito Hattori, Yoshihiro Sakata, Yoshiyuki Soeda, Akihiko Takashima, Hideaki Yoshimura, Gen Matsumoto, Hiroko Bannai
"Inducing aggresome and stable tau aggregation in Neuro2a cells with an optogenetic tool"
URL:https://doi.org/10.2142/biophysico.bppb-v21.0023
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- Abstract
- Tauopathy is a spectrum of diseases characterized by fibrillary tau aggregate formation in neurons and glial cells in the brain. Tau aggregation originates in the brainstem and entorhinal cortex and then spreads throughout the brain in Alzheimer’s disease (AD), which is the most prevalent type of tauopathy. Understanding the mechanism by which locally developed tau pathology propagates throughout the brain is crucial for comprehending AD pathogenesis. Therefore, a novel model of tau pathology that artificially induces tau aggregation in targeted cells at specific times is essential. This study describes a novel optogenetic module, OptoTau, which is a human tau with the P301L mutation fused with a photosensitive protein CRY2olig, inducing various forms of tau according to the temporal pattern of blue light illumination pattern. Continuous blue light illumination for 12 h to Neuro2a cells that stably express OptoTau (OptoTauKI cells) formed clusters along microtubules, many of which eventually accumulated in aggresomes.