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An article with the title "Quantitative and kinetic single-molecule analysis of DNA unwinding by Escherichia coli UvrD helicase" by Hiroaki Yokota is published.

2022 March 25 BPPB

A following article is published in "Biophysics and Physicobiology".

Hiroaki Yokota
"Quantitative and kinetic single-molecule analysis of DNA unwinding by Escherichia coli UvrD helicase"

【Significance】
Helicases are nucleic acid-unwinding enzymes involved in the maintenance of genome integrity. Despite the high structural homology among superfamily 1 non-hexameric helicases, two opposing models have been proposed for their functional units: monomeric and dimeric-helicase models. This paper reviews past and recent studies on the Escherichia coli SF1 helicase UvrD. The author's single-molecule direct visualization of a UvrD mutant lacking the C-terminal 40 amino acids (UvrDΔ40C), which was used to propose the monomer model, provided evidence for the dimer model. Thus, single-molecule direct visualization of nucleic acid-binding proteins can provide quantitative and kinetic information to reveal their fundamental mechanisms.

Biophysics Physicobiology, 2022, Volume 19, Article ID: e190006



URL:https://doi.org/10.2142/biophysico.bppb-v19.0006



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