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2015年09月09日 掲載 (Published 09/09/2015)


「Biophysics and Physicobiology」にTakumi Koshiba "Protein-protein interactions of mitochondrial-associated protein via bioluminescence resonance energy transfer"を掲載

日本生物物理学会欧文誌[Biophysics and Physicobiology]に以下の論文が新規掲載されました。

Takumi Koshiba
”Protein-protein interactions of mitochondrial-associated protein via bioluminescence resonance energy transfer”

【Significance】
Monitoring of protein-protein interactions in live cells

Protein-protein interactions are essential biological reactions occurring at inter- and intra-cellular levels. Bioluminescence resonance energy transfer (BRET), which is based on an enzymatic activity of luciferase, is a powerful and ideal tool for evaluating protein-protein interactions, especially in live cells. The combination of the BRET system and biomolecular fluorescence complementation would provide us a better understanding of the hetero-oligomeric structural states of protein complexes. Here we discuss the application of BRET to the protein-protein interactions of mitochondrial-associated proteins and discuss its physiological relevance.


Biophysics and Physicobiology, Vol.12, pp. 31-35

URL:http://doi.org/10.2142/biophysico.12.0_31