ニュース

2015年12月28日 掲載 (Published 12/28/2015)


「Biophysics and Physicobiology」に Daichi Yamada et al. “Structural role of two histidines in the (6-4) photolyase reaction”を掲載

日本生物物理学会欧文誌[Biophysics and Physicobiology]に以下の論文が新規掲載されました。

Daichi Yamada, Tatsuya Iwata, Junpei Yamamoto, Kenichi Hitomi, Takeshi Todo, Shigenori Iwai, Elizabeth D. Getzoff and Hideki Kandori 
“Structural role of two histidines in the (6-4) photolyase reaction”

【Significance】
Structural role of two histidines in the (6-4) photolyase reaction

(6-4) photolyases is a DNA repair enzyme by light. Previous mutation study showed the importance of two histidines, H354 and H358 for Xenopus (6-4) photolyase, for repair reaction. Based upon highly sensitive FTIR analysis, we report that both H354A and H358A mutants still maintain their repair activity. Similar difference FTIR spectra between the wild-type and mutant proteins suggest a common mechanism of repair, and a decrease in volume by mutation is possibly compensated by the addition of water molecule(s). Such a modified environment is sufficient for function, while two histidines significantly raises the efficiency.


Biophysics and Physicobiology, Vol.12, pp. 139-144

URL:http://doi.org/10.2142/biophysico.12.0_139