ニュース

「Biophysics and Physicobiology」にNakanishi et al. “Cryo-EM studies of the rotary H+-ATPase/synthase from Thermus thermophilus”を掲載

2019年09月06日 学会から

日本生物物理学会欧文誌[Biophysics and Physicobiology]に以下の論文が新規掲載されました。

Atsuko Nakanishi, Jun-ichi Kishikawa, Kaoru Mitsuoka, Ken Yokoyama “Cryo-EM studies of the rotary H+-ATPase/synthase from Thermus thermophilus

【Significance】
Cryo-EM studies of V/A type H+-rotary ATPase

ATP hydrolysis/synthesis in the soluble domain of proton-translocating rotary ATPases is coupled with proton flux across the membrane domain via a rotation of the common central rotor complex against the surrounding peripheral stator apparatus. Structures of the complete V/A type H+-rotary ATPase have been revealed at a near atomic resolution level using cryogenic electron microscopy (cryo-EM). In this review article, we will describe the information about specimen preparation to improve the image quality and cryo-EM single particle analysis to overcome the intrinsic conformational heterogeneity of the Tth V/A-ATPases.

Biophysics and Physicobiology, Vol.16, 140-146
URL: https://doi.org/10.2142/biophysico.16.0_140


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