ニュース

「Biophysics and Physicobiology」にKaneshiro et al. “Second harmonic generation polarization microscopy as a tool for protein structure analysis”を掲載

2019年09月20日 学会から

日本生物物理学会欧文誌[Biophysics and Physicobiology]に以下の論文が新規掲載されました。

Junichi Kaneshiro, Yasushi Okada, Tomohiro Shima, Mika Tsujii, Katsumi Imada, Taro Ichimura, Tomonobu M. Watanabe “Second harmonic generation polarization microscopy as a tool for protein structure analysis”

【Significance】
Structural analysis by SHG microscopy

Label-free optical modality using the second-harmonic generation (SHG) microscope reveals conformational variation of proteins and their dynamics under physiological conditions. A conformational change of microtubules (MTs) is detected through the SHG tensor analysis. A phenomenological model for an MT suggests that the electric dipole moment in each tubulin slightly tilts outward on taxol binding. A time development of an SHG tensor of a crystal of photo-switchable fluorescent protein Kohinoor is successfully observed both in activation and inactivation processes. Compared with two-photon fluorescence measurement, the polarization dependence of SHG intensity shows unique features indicating structural change of Kohinoor on photo switching.

Biophysics and Physicobiology, Vol.16, 147-157
URL:https://doi.org/10.2142/biophysico.16.0_147


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