ニュース

2016年07月15日 掲載 (Published 07/15/2016)


「Biophysics and Physicobiology」に 〈Special Issue: "Protein-Ligand Interactions"〉 Masayuki Oda et al. “Effects of substrate conformational strain on binding kinetics of catalytic antibodies”を掲載

日本生物物理学会欧文誌[Biophysics and Physicobiology]に以下の論文が新規掲載されました。

Masayuki Oda, Takeshi Tsumuraya, Ikuo Fujii
“Effects of substrate conformational strain on binding kinetics of catalytic antibodies”

【Significance】
The slow association rate in protein-ligand interactions is attributed to the ligand conformational strain

The binding kinetics of catalytic antibodies, 6D9, 9C10, and 7C8, with substrate and transition-state analogue were determined and correlated with structural and thermodynamic information. The efficient catalytic antibody, 6D9, binds the substrate, changing the conformation to an unstable twisted conformation with enthalpic strain. The present analysis showed that the association rate for 6D9 binding to the substrate was much lower than that to transition-state analogue, whereas those for 9C10 and 7C8 binding were similar to those to transition-state analogue. The correlation of conformational strain with binding kinetics would widely apply for other protein-ligand interactions.


Biophysics and Physicobiology, Vol.13, pp. 135-138

URL:https://www.jstage.jst.go.jp/article/biophysico/13/0/13_135/_article