ニュース

2016年10月07日 掲載 (Published 10/07/2016)


「Biophysics and Physicobiology」にYasuhiro Onoue et al. “Domain-based biophysical characterization of the structural and thermal stability of FliG, an essential rotor component of the Na+-driven flagellar motor”を掲載

日本生物物理学会欧文誌[Biophysics and Physicobiology]に以下の論文が新規掲載されました。

Yasuhiro Onoue, Rei Abe-Yoshizumi, Mizuki Gohara, Yuuki Nishino, Shiori Kobayashi, Yasuo Asami and Michio Homma “Domain-based biophysical characterization of the structural and thermal stability of FliG, an essential rotor component of the Na+-driven flagellar motor”

【Significance】
Domain-based biophysical characterization of structural and thermal stability of FliG in Na+-driven flagellar motor

Bacteria can swim by rotating their flagella. Interaction between the stator protein and the rotor protein makes the rotational driving force. FliG is the most important component of the rotor for the torque generation in the flagellar motor. Here, we purified FliG and its fragmants from Vibrio alginolyticus, which has a polar flagellum to characterize the biochemical/biophysical properties by gel-filtration chromatography, circular dichroism spectroscopy, and differential scanning calorimetry. The results suggest that FliG has at least two conformational states and that each domain of FliG is responsible for the denaturation. These provide information on the basic characteristics of FliG.


Biophysics and Physicobiology, Vol.13, pp. 227-233

URL:https://www.jstage.jst.go.jp/article/biophysico/13/0/13_227/_article