「Biophysics and Physicobiology」にKeiichi Kojima et al. “Mutational analysis of the conserved carboxylates of anion channelrhodopsin-2 (ACR2) expressed in Escherichia coli and their roles in anion transport”を掲載

2018年09月10日 学会誌

日本生物物理学会欧文誌［Biophysics and Physicobiology］に以下の論文が新規掲載されました。

Keiichi Kojima, Hiroshi C. Watanabe, Satoko Doi, Natsuki Miyoshi, Misaki Kato, Hiroshi Ishikita, Yuki Sudo “Mutational analysis of the conserved carboxylates of
anion channelrhodopsin-2 (ACR2) expressed in Escherichia coli and their roles in anion transport”

【Significance】
Combined experimental and theoretical approaches provide functionally important carboxylates and its anion transport mechanism of ACR2

Anion channelrhodopsin-2 (ACR2) was recently identified from the eukaryotic alga Guillardia theta as a light-gated anion channel that consists of seven-transmembrane helices and a retinal chromophore. Here we investigated the roles of six conserved carboxylates in the anion transport activity of ACR2 using an Escherichia coli expression system. Two of those carboxylates, E159 and D230, were identified as functionally important residues. A homology model structure of ACR2 showed that E159 and D230 have significant interactions with R129 and the retinal chromophore, respectively. These combined experimental and theoretical approaches provide molecular insights into the anion transport mechanism of ACR2.


Biophysics and Physicobiology, Vol.15, pp. 179-188

URL:https://www.jstage.jst.go.jp/article/biophysico/15/0/15_179/_article/

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