「Biophysics and Physicobiology」に Shingo Wakao, Noriko Saitoh, Akinori Awazu による "Mathematical model of structural changes in nuclear speckle" をJ-STAGEの早期公開版として掲載
2023年04月27日 学会誌
日本生物物理学会欧文誌[Biophysics and Physicobiology]に以下の論文が早期公開されました。
Shingo Wakao, Noriko Saitoh, Akinori Awazu
"Mathematical model of structural changes in nuclear speckle"
URL:https://doi.org/10.2142/biophysico.bppb-v20.0020
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- Abstract
- Nuclear speckles are nuclear bodies consisting of populations of small and irregularly shaped droplet-like molecular condensates that contain various splicing factors. Recent experiments have revealed the following structural features of nuclear speckles: (I) Each molecular condensate contains SON and SRRM2 proteins, and MALAT1 non-coding RNA surrounds these condensates; (II) During normal interphase of the cell cycle in multicellular organisms, these condensates are broadly distributed throughout the nucleus. In contrast, when cell transcription is suppressed, the condensates fuse and form strongly condensed spherical droplets; (III) SON is dispersed spatially in MALAT1 knocked-down cells and MALAT1 is dispersed in SON knocked-down cells because of the collapse of the nuclear speckles. However, the detailed interactions among the molecules that are mechanistically responsible for the structural variation remain unknown. In this study, a coarse-grained molecular dynamics model of the nuclear speckle was developed by considering the dynamics of SON, SRRM2, MALAT1, and pre-mRNA as representative components of the condensates. The simulations reproduced the structural changes, which were used to predict the interaction network among the representative components of the condensates.
URL:
https://doi.org/10.2142/biophysico.bppb-v20.0020