ニュース

「Biophysics and Physicobiology」に Manabu Hori, Takashi Tominaga, Masaki Ishida, Mutsumi Kawano による "RNA interference reveals the escape response mechanism of Paramecium to mechanical stimulation" をJ-STAGEの早期公開版として掲載

2023年05月31日 学会誌

日本生物物理学会欧文誌[Biophysics and Physicobiology]に以下の論文が早期公開されました。

Manabu Hori, Takashi Tominaga, Masaki Ishida, Mutsumi Kawano
"RNA interference reveals the escape response mechanism of Paramecium to mechanical stimulation"

URL:https://doi.org/10.2142/biophysico.bppb-v20.0025


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Abstract
In Paramecium, a mechanical stimulus applied to the posterior portion of the cell causes a transient increase in membrane permeability to potassium ions, transiently rendering the membrane in a hyperpolarized state. Hyperpolarization causes a transient increase in Cyclic adenosine monophosphate (cAMP) concentration in the cilia, resulting in a transient fast-forward swimming of the cell. Schultz and coworkers (1992) reported that a unique adenylate cyclase (AC)-coupled potassium channel is involved in the reaction underlying this response, which is known as the “escape response.” However, the AC responsible for this reaction remains to be identified. Moreover, the molecular linkage between mechanoreception and AC activation has not been elucidated adequately. Currently, we can perform an efficient and simple gene-knockdown technique in Paramecium using RNA interference (RNAi). Paramecium is one of the several model organisms for which whole-genome sequences have been elucidated. The RNAi technique can be applied to whole genome sequences derived from the Paramecium database (ParameciumDB) to investigate the types of proteins that elicit specific biological responses and compare them with those of other model organisms. In this review, we describe the applications of the RNAi technique in elucidating the molecular mechanism underlying the escape response and identifying the AC involved in this reaction. The findings of this study highlight the advantages of the RNAi technique and ParameciumDB.



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