「Biophysics and Physicobiology」に Junko Nakai, Kengo Namiki, Yuki Totani, Shigeki Yasumasu, Teruki Yoshimura, Takashi Aoki, Etsuro Ito による "Changes in protein phosphorylation by insulin administration in the central nervous system of the gastropod mollusk Lymnaea stagnalis" をJ-STAGEの早期公開版として掲載
2023年10月12日 学会誌
日本生物物理学会欧文誌[Biophysics and Physicobiology]に以下の論文が早期公開されました。
Junko Nakai, Kengo Namiki, Yuki Totani, Shigeki Yasumasu, Teruki Yoshimura, Takashi Aoki, Etsuro Ito
"Changes in protein phosphorylation by insulin administration in the central nervous system of the gastropod mollusk Lymnaea stagnalis"
URL:https://doi.org/10.2142/biophysico.bppb-v20.0038
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- Abstract
- In the gastropod mollusk Lymnaea stagnalis, insulin-like peptides in the central nervous system (CNS) control behavioral changes associated with associative learning. Insulin administration to the Lymnaea CNS enhances the synaptic plasticity involved in this type of learning, but it has remained unclear which molecules in the insulin response cascade are involved. Here, to advance a comprehensive analysis, we used two-dimensional electrophoresis and comparative quantitative mass spectrometry to perform a protein analysis investigating the CNS molecules that respond to insulin administration. Our results revealed increased phosphorylation of AKT and RICTOR in the PI3K/AKT/mTOR signaling cascade and cytoskeleton-related proteins. Although it was expected that the molecules in the PI3K/AKT/mTOR signaling cascade were phosphorylated by insulin administration, our findings confirmed the correlation between insulin-induced phosphorylation of cytoskeleton-related proteins strongly involved in the synaptic changes and learning and memory mechanisms. These results contribute to elucidate the relationship between the insulin response and learning and memory mechanisms not only in Lymnaea but also in various invertebrates and vertebrates.
URL:
https://doi.org/10.2142/biophysico.bppb-v20.0038