「Biophysics and Physicobiology」に Daichi Yamada et al. “Structural role of two histidines in the (6-4) photolyase reaction”を掲載

2015年12月28日 学会誌

日本生物物理学会欧文誌［Biophysics and Physicobiology］に以下の論文が新規掲載されました。

Daichi Yamada, Tatsuya Iwata, Junpei Yamamoto, Kenichi Hitomi, Takeshi Todo, Shigenori Iwai, Elizabeth D. Getzoff and Hideki Kandori　
“Structural role of two histidines in the (6-4) photolyase reaction”

【Significance】
Structural role of two histidines in the (6-4) photolyase reaction

(6-4) photolyases is a DNA repair enzyme by light. Previous mutation study showed the importance of two histidines, H354 and H358 for Xenopus (6-4) photolyase, for repair reaction. Based upon highly sensitive FTIR analysis, we report that both H354A and H358A mutants still maintain their repair activity. Similar difference FTIR spectra between the wild-type and mutant proteins suggest a common mechanism of repair, and a decrease in volume by mutation is possibly compensated by the addition of water molecule(s). Such a modified environment is sufficient for function, while two histidines significantly raises the efficiency.


Biophysics and Physicobiology, Vol.12, pp. 139-144
URL: http://doi.org/10.2142/biophysico.12.0_139

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